Current Issue : January - March Volume : 2016 Issue Number : 1 Articles : 6 Articles
Objectives. To carry out a descriptive analysis of the expression of the EP3 receptors of PGE2 in different histological grades of OSCC\nand adjacent normal epithelium. Material and Methods. A total of 46 patients presenting with various histological subtypes and\ngrades of OSCC were recruited from Maxillofacial Surgery Department of Nishtar Institute of Dentistry Multan. Microscopically\ntumour subtyping and histological grading according to Anne roth�s grading system were carried out. Immunohistochemical\nstaining with rabbit polyclonal EP3 receptor antibody was performed and sections were scored for intensity and proportion of\npositive adjacent squamous epithelial and tumour cells. Results. Out of 46 patients ...
Cutaneous leishmaniases (CL) are endemic in Morocco. They are common in the human population in different localities such\nas Aichoun in Sefrou province, Morocco. This study was carried out in Aichoun locality from April to October 2012 in order to\nstudy the spatiotemporal trends of the main Leishmania phlebotomine vectors in this focus. Overall, 1171 sand flies, belonging to\nfour species, were collected by sticky traps. Phlebotomus sergenti was the predominant species (78.4%) followed by Ph. perniciosus\n(10.5%), Ph. papatasi (7.94%), and Ph. longicuspis (3.16%). Sand flies were active during 6 months (Mayââ?¬â??October). Ph. sergenti, Ph.\nperniciosus, and Ph. papatasi displayed a bimodal distribution with a first peak in July and a second peak in September, while Ph.\nlongicuspis showed a monophasic trend with a peak in August. The high abundance and the lengthy period of activity of Ph. sergenti\nand Ph. perniciosus, vectors of L. tropica and L. infantum, respectively, are a cause for concern as they indicate the high potential\nrisk of Leishmania transmission in the studied areas....
Aim of this study was to investigate heart valve calcification process by different biomineralogical techniques to provide\nmorphological and chemical features of the ectopic deposit extracted from patients with severe mitral and aortic valve stenosis,\nto better evaluate this pathological process. Polarized light microscopy and scanning electron microscopy analyses brought to light\nthe presence of nodular and massive mineralization forms characterized by different levels of calcification, as well as the presence\nof submicrometric calcified globular cluster, micrometric cavities containing disorganized tissue structures, and submillimeter\npockets formed by organic fibers very similar to amyloid formations. Electron microprobe analyses showed variable concentrations\nof Ca and P within each deposit and the highest content of Ca and P within calcified tricuspid aortic valves, while powder X-ray\ndiffraction analyses indicated in the nanometer range the dimension of the pathological bioapatite crystals. These findings indicated\nthe presence of highly heterogeneous deposits within heart valve tissues and suggested a progressive maturation process with\ncontinuous changes in the composition of the valvular tissue, similar to themultistep formation process of bone tissue.Moreover the\nmicrometric cavities represent structural stages of the valve tissue that immediately precedes the formation of heavily mineralized\ndeposits such as bone-like nodules....
Oesophageal cancer is the eighth commonest form of cancer world-wide with variable incidence rates across different geographical regions. Squamous cell type is the commonest histological type in developing countries. This study aimed to determine the pathological subtypes of oesophageal cancer and the probable roles of some markers in the metastatic potential of uncommon subtypes. The study Proposal was approved by the institutional review board. Clinical data and histopathology results of 55 resection specimens from oesophageal cancer cases were reviewed. Two uncommon tumours, a basaloid squamous carcinoma and a squamous cell carcinoma that invaded the stomach were analyzed by immunohistochemistry for stemness and pathogenesis. All specimens received were stage T3N2 or T3N3 and had carbon pigment in the draining lymph nodes. The most common histological type was squamous cell carcinoma of different degrees of differentiation (74%). Adenocarcinoma was less common and was reported in 14.6% of the cases. The basaloid squamous carcinoma consisted of well differentiated squamous cells and basaloid spindle cells. Both the squamous and basaloid components were positive for P63. Basaloid cells were positive for CD44, weakly positive for OCT4 and negative for E-Cadherin. This part metastasised to regional lymph nodes. The oesophageal carcinoma that invaded the stomach was positive for CD44 but negative for E-Cadherin. The CD44 and E-Cadherin changes described in the basaloid squamous carcinoma were in the gastric component which metastasised to abdominal lymph nodes.\n\nConclusion: Squamous cell carcinoma of different degrees of differentiation is the commonest type of oesophageal cancer in Sudan with variations in male: female ratios. Over expression of CD44 and down regulation E-Cadherin are probably associated with aggressiveness and metastasis....
Immunohistochemistry is important for the accurate diagnosis of basal cells in atypical glandular proliferations of the prostate.\np40, an isoform of p63, may be an adjunct to a marker panel in this setting. Biopsies of 68 patients were analyzed by\nimmunohistochemistry using antibodies to 34betaE12 and p40. Basal cell staining was classified as negative, partial (<60%), or\ndiffuse (�60%); irregular staining was defined as discordant staining patterns. In acinar proliferations (...
Background. Radiofrequency ablation (RFA) has been recently advocated as an effective technique for the treatment of symptomatic\nbenign thyroid nodules. It is not known to what extent it may affect any subsequent thyroid surgery and/or histological diagnosis.\nMaterials and Methods. RFA was performed on 64 symptomaticThy2 nodules (benign nodules) and 6 symptomaticThy3 nodules\n(follicular lesions/follicular neoplasms). Two Thy3 nodules regrew after the procedure, and these patients accepted to undergo\na total thyroidectomy. Here we present how RFA has affected the operation and the final pathological features of the surgically\nremoved nodules. Results and Conclusions. RFA is effective for the treatment of Thy2 nodules, but it should not be recommended\nas first-line therapy for the treatment of Thy3 nodules (irrespective of their mutational status), as it delays surgery in case of\nmalignancy. Moreover, it is unknown whether RFA might promote residual tumor progression or neoplastic progression of Thy3\nlesions. Nevertheless, here we show for the first time that one session of RFA does not affect subsequent thyroid surgery and/or\nhistological diagnosis....
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